I need pointers on how to preserve cells before making spheroplasts and
cell fractionation. Basically, I have to do an 8-12 hr. incubation of
cells before harvesting for spheroplast formation and cell disruption.
Rather than schedule 16 hr. days, I'm thinking that there must be a way to
harvest cells, freeze them off for example, and then generate spheroplasts
for homogenization. Any thoughts or experiences would be appreciated.