I am wondering if there is anybody out there who has a good protocol for
selecting kanamycin-MX transformants. I am trying to epitope-tag my
favorite ORF with 3xHA or myc using one of the Longtine vectors, and I
don't see a high rate of transformants on my geniticin selective plates.
Is there a 'trick' with transformation protocol using the KanMX system?
Many thanks for any info or hints.
tjandra at darwin.ucsc.edu