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Good Vector/Kit system for expression of highly basic proteins in Yeast?

Graham Dellaire, Ph.D. Graham.Dellaire at hgu.mrc.ac.uk
Wed Apr 7 11:02:37 EST 1999

Hello All, 

I am playing with the idea of expressing a fairly basic protein in yeast
rather than E. coli as my gene sequence contains many rare Arginine
codons (AAG, AGA) and I am having trouble expressing it.  Does anyone
know of a good expression system in yeast (from experience) that I could
use to produce mg quantities of protein for antibody production??

Thanks in advance, all suggestions wellcome.


Graham Dellaire
Graham Dellaire, Ph.D.

MRC Human Genetics Unit
Western General Hospital
Crewe Road
Edinburgh, UK

Fax: +44 (0)131 343 2620   
Phone: +44 (0)131 332 2471

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