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PCR Mediated Gene Replacement in S.pombe?

Nick nowen at bioch.ox.ac.uk
Fri Nov 20 15:38:56 EST 1998

Dear All,

I am interested in attempting gene replacement using PCR mediated
techniques using a URA4 marker gene. 

A while ago someone posted a URL of a nice paper on this but due to a
system crash I cannot find this information.

If anyone has the posting/URL for a paper on PCR mediated gene replacement
or has any suggestions I would appreciate it greatly

Kindest regards

Nicholas Owen

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