You can check ATCC catalogue online. They have the plasmid you want with
different markers on it.
Joe Mymryk wrote:
> I am looking for a plasmid with the TEF1 promoter in it that I could use
> to drive a high level of expression of a heterologous gene in S.c.
> Would anyone be willing to send me something like this? Markers are not
> an issue if I can cut it out of the plasmid.
>> I saw Björn Johansson's posting on the subject on 25 Sep 1998,
>> "Does anyone know where I can get hold of an expression plasmid for
> S.cerevisiae with the TEF1 promotor (translational elongation factor
> EF-1 alpha) ? Thanks in advance for any answers !"
>> but couldn't get any email through to him to see if he had obtained what
> he was looking for.
>> Joe Mymryk
>> London Regional Cancer Centre
> 790 Commissioners Rd. East
> London, Ontario, Canada N6A 4L6
>> Phone: (519) 685-8300 ext. 53012 Fax: (519) 685-8616
> Email: jmymryk at julian.uwo.ca