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Chromosomal integration

Björn Johansson bjorn.johansson at tmb.lth.se
Fri Nov 13 06:55:48 EST 1998


Hello,

I have a theoretical question regarding chromosomal integration in yeast
(S.c.).
I would like to design a vector with PCR mediated integration ( just
like PCR mediated gene disruption).
This means 30-40 bp of homology on each side. Could my vector contain
sequences that are already present
in the yeast genome ? Would there be a large number of vectors
integration at the wrong positions ?

Sincerely
Björn Johnasson







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