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Transformation Hints

Michael Forte forte at ohsu.EDU
Thu May 28 12:34:26 EST 1998


I know that this has been discussed here but I am trying to find hints to
up our transformation efficiency.  Right now, we are hovering around
25,000 per ug of DNA.  I have heard reports of over a million per ug but
even using the now famous TRAFO method, we were not able to improve things
much, if at all.  I know from experience that transformation efficiency is
very strain dependent but we would like to at least get 100,000 per ug
reproducibly, mainly for library screens.  Any hints? Electroporation

Mike Forte

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