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yeast homogenates

Orlando Morais omorais at mail.telepac.pt
Mon May 25 01:35:26 EST 1998


Chris Doering wrote in message <6jsdn8$i3i at net.bio.net>...
>klennon at ACS1.BU.EDU wrote:
>>
>> Hi.
>>
>> I am looking for a protocol to homogenize yeast cells for Western blot
>> analyses that does not involve using a bead beater. I just went through a
>> lovely protocol using glass beads, only to have my cells
>> completely intact at the end! I have routinely done RNA isolations from
yeast
>> using glass beads, so I know you have to vortex the hell out of them to
break
>> them, but it didn't work well at all with this protocol.
>>
>> Any help would be greatly appreciated!
>>
>> Sincerely,
>> Kelley Lennon, D.Sc.


I have used a glass rod  (that fit an eppendorf tube) with glass beads at a
medium speed. It really works for protein extracts, and usaually i obtained
almost 90% of breaked cells.

Good look.
omorais at mail.telepac.pt




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