Hi !
I'm new to the yeast field and I was delighted to find this
newsgroup! I'm trying to set up a genetic screen to look for mutants in
the TATA box binding protein that would enhance transcription of the
HIS3 gene. I'm using 3-amino-1,2,4-triazole (AT) to cut down the
background growth of my strain but I'm running into problems: at low
concentrations (0.2mM-10mM) I see a large number of microcolonies and at
higher concentrations of AT (20mM-80mM), I don't see any colonies at
all.
My question is; Is there any particular source from where the Yeast
community obtains its AT: We're using AT from Sigma and I noticed a mail
posted in 1995 to this newsgroup with the same question (i.e., whether
AT from Sigma could be used) but couldn't find any answer to it.
I've also heard that AT takes a long time to dissolve in water but the
AT we use (from Sigma) dissolves quite rapidly.
Can anyone help me?
Thanks!
Lata Chitikila
452 North Frear Laboratory,
Department of Biochemistry and Molecular Biology,
The Pennsylvania State University,
University Park, PA16802
cxc34 at psu.edu
