Fergus Doherty wrote:
>> Most protocols seem to call for Zymolyase 100 for spheroplasting, but can
> anyone recommend a cheaper alternative? How about Sigma Lyticase or
> Glusulase?
>> --
> Fergus Doherty,
> Dept Biochemistry,
> Nottingham University,
>>Fergus.Doherty at nottingham.ac.uk> 0115 970 9366 (74-41366 internal)
Sigma Lyticase or Zymolase 20T works well for me. I use 2 mg per gram of
wet cell weight. 90' at RT or 60' at 37 degrees, rocking gently.
Brett Burkholder
Dean Danner Lab
Emory University
Department of Genetics
Atlanta, GA 30322
