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Alternatives to Zymolyase 100?

Chris Doering cdoerin at emory.edu
Tue May 19 11:59:07 EST 1998


Fergus Doherty wrote:
> 
> Most protocols seem to call for Zymolyase 100 for spheroplasting, but can
> anyone recommend a cheaper alternative?  How about Sigma Lyticase or
> Glusulase?
> 
> --
> Fergus Doherty,
> Dept Biochemistry,
> Nottingham University,
> 
> Fergus.Doherty at nottingham.ac.uk
> 0115 970 9366 (74-41366 internal)


Sigma Lyticase or Zymolase 20T works well for me. I use 2 mg per gram of
wet cell weight.  90' at RT or 60' at 37 degrees, rocking gently.

Brett Burkholder
Dean Danner Lab
Emory University
Department of Genetics
Atlanta, GA 30322




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