I have just purchased a "Genestorm" vector from Invitrogen. For those of
you who are not aware of this, you may want to check it out... it has a
C-terminal V5 antigen tag and His6 tag on the expressed yeast ORF, under
GAL 1 and T7 promoters, with origins and markers for yeast and bacterial
expression. They are planning to make such constructs for all the
identified yeast ORFs.
Does anyone have experience using one of these plasmids? Specifically,
I'm interested in level of expression of the ORF, interference with
mitochondrial targeting from the tags, and V5 antibody cross-reactivity.
Any help would be appreciated.
Brett Burkholder
Dean Danner Lab
Emory University
Department of Genetics
Atlanta, GA 30322