In the following reference:
Methods Enzymol 1997;283:459-471
Use of green fluorescent protein for intracellular protein
localization in living fission yeast cells.
Nabeshima K, Saitoh S, Yanagida M
they refer to a vector of expression in S.pombe that has the LEU2
marker and nmt promoter and stops. The cloning site has SalI, BamHI
and NotI restriction sites which is absolutley perfect for the linker
primers I have got for a number of cDNA's that I have amplified. By
using these sites it is possible to use the vector to 3' tag the cDNA
with the GFP ORF.
I was wondering if anyone out there has this pGP110 vector for
expression in yeast?
Please email if you have
nick_owen at wols.demon.co.uk
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