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PCR-mediated gene disrupt in pombe?

Rich Maraia rm123g at nih.gov
Thu May 14 19:57:04 EST 1998


I am a novice at S. pombe mol. genetics and would like to know if it is
feasible to use the PCR Procedure to make a gene disruption in pombe. 
Briefly, the method is to make chimeric primers that include ~80 nt of
gene-specific sequence attached to sequence matching the marker gene of
interest (e.g., ura4+); after PCR, transformation should lead to
homologous recombination at the gene-specific locus.

Thank you in advance,
Rich Maraia
NIH, USA




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