I am a novice at S. pombe mol. genetics and would like to know if it is
feasible to use the PCR Procedure to make a gene disruption in pombe.
Briefly, the method is to make chimeric primers that include ~80 nt of
gene-specific sequence attached to sequence matching the marker gene of
interest (e.g., ura4+); after PCR, transformation should lead to
homologous recombination at the gene-specific locus.
Thank you in advance,