I am doing a three-hybrid screening in yeast. After
retransformation of the rescued plasmids into yeast
I am getting unexpected results, namely that the
level of activation is lower and now it seems
unspecific. This is quite surprising to me, since
I have isolated twice the same cDNA, I mean, two
of my positives beared the same cDNA insert, what
I had interpreted as a good sign.
Any help or comment will be highly appreciated.
Many thanks in advance.
Jose L. Carrasco
jlcarrasco at ibmcp.upv.es