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In article <3542C928.DA7 at ucla.edu>, Diana Go Young <dyoung at ucla.edu> wrote:
>Hi everyone!
>>Does anyone have information on the following concerning yeast clathrin
>heavy chain?
>>1. Was the protein or gene first isolated? How was it isolated?
>2. Has a promoter for this gene been characterized?
>>Thanks.
>>Diana Young
>>
>From http://genome-www.stanford.edu/cgi-bin/SGD/search
Locus_info: Other_name YGL206C
Gene_class CHC
Gene_Info CHC1
Description homologous to clathrin heavy chain
Gene_product presumed vesicle coat protein
Phenotype Null mutant is viable, but is
slow-growing and shows defects in
mating, sporulation and vesicle
ultrastructure (however it shows little
or no defect in secretion); null mutants
easily become inviable due to second
site mutations in a number of unlinked
genes such as SCD1 and CDL1
Position_info: Chromosome VII
Map VII Position -133
ORF_name YGL206C
Sequence_info: ORF_sequence YGL206C [MIPS ORF Info|Seq & Display]
Sequence YGL206C [Retrieve DNA/Protein]
X52900_1.cds [Retrieve DNA/Protein]
Z72728_1.cds [Retrieve DNA/Protein]
Protein_info: YPD CHC1 [YPD @ Proteome]
SwissProt CLH_YEAST P22137 [Entrez Protein|Related Protein
Sequences|SwissPROT]
PIR A36349 [PIR-DE|PIR-JP|PIR-US] A36349
Sacch3D YGL206C [Sacch3D]
Reference Payne, G.S., et al. (1987) Genetic and
biochemical characterization of
clathrin-deficient Saccharomyces
cerevisiae. Mol Cell Biol 7:3888-3898
Munn, A.L., et al. (1991) Viability of
clathrin heavy-chain-deficient
Saccharomyces cerevisiae is compromised
by mutations at numerous loci:
implications for the suppression
hypothesis. Mol Cell Biol 11:3868-3878
Payne, G.S. and R. Schekman (1985) A
test of clathrin function in protein
secretion and cell growth. Science
230:1009-1014
Lemmon, S.K. and E.W. Jones (1987)
Clathrin requirement for normal growth
of yeast. Science 238:504-509
Mapping_data chc1-cdc55-399
chc1-cdc43-400
ade5,7-CHC1-2406
CHC1-kex1-2408
ade5,7-CHC1-2409
CHC1-lys5-2411
SGDID L0000317
