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In article <6dl3rc$nqj at net.bio.net>, Michael Forte <forte at ohsu.EDU> wrote:
>Hi,
>>I know this has been discussed here before, but can anyone give me some
>hints as to how to make selection on FOA plates more stringent. Right
>now, the difference in growth on minimal plates of ura+ and ura- strains
>is minimal. Are there tricks to make this difference more pronounced?
>Thanks in advance.
>>Mike Forte
>
In our lab we replicate plates to 4-5 "junk" plates before replicating to
FOA, the Idea is to get a monolayer of cells on the velvet before going to
FOA this seems to work OK.
Aidan
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