IUBio

Collection of amplified yeast ORFs or primer pairs

Rob Kirkpatrick kirkpat at ?cc.umanitoba.ca?
Mon Mar 9 14:50:36 EST 1998


In article <6doj6t$eav at net.bio.net>, Mathieu KLEIN
<klein at mpimp-golm.mpg.de> wrote:

> Dear Saccharonetters,
> 
> Does anyone know a place where I could get one of the following products:
> 1. a set of amplified yeast ORFs (complete coding region),
> 
> or
> 2. a set of primer pairs to amplify each ORF.
> 
> 
> We would have to deal with a few hundreds of ORFs, which of course
> represents a significant cost factor if we have to design and synthetize the
> primers.
> So far I know, I could get this by Research Genetics for a fair price, but
> they do NOT
> communicate any primer sequence informations.
> Since we have to prepare some (N- and/or C-terminal) fusion protein
> constructs, these products are unusable for our purposes !!!

Research Genetics does have the primer sequences listed somewhere - I
believe it is on their web page.  It's a little obscure to find, but it is
out there...

Good luck.

-- 

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