An example of a a purification of a his-tagged membrane protein is
described in Loddenkötter et al. (1993) PNAS 90; 2155-2159.
Personally I have good results with his-tagged intracellular and
secretory protein in yeast.
Good luck,
Georg
>> Hey,
>> Can anyone send me or reference a procedure concerning the purification
>> of a 6X-His tag protein from S. cerevisiae on a Ni -column. I'd love to
>> be able to lyse the cells and pour the extract directly over the Ni-
>> column. The tagged protein is a membrane spanning protein so the
>> procedure will need to tolerate a detergent of types. Many thanks.
>>>> Antony Cooper
>>>>>>
