before you get too far into this idea, go to the yeast genome
database and do a search for the HHHHHH amino acid sequence. this
will show you just how many naturally His6 tagged yeast proteins will
be coming down in your column.
Antony Cooper wrote:
> Can anyone send me or reference a procedure concerning the purification
> of a 6X-His tag protein from S. cerevisiae on a Ni -column. I'd love to
> be able to lyse the cells and pour the extract directly over the Ni-
> column. The tagged protein is a membrane spanning protein so the
> procedure will need to tolerate a detergent of types. Many thanks.
>> Antony Cooper