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2-hybrid: inhibitor of LEU2 ?

Dr. Rainer Duden rd217 at hermes.cam.ac.uk
Mon Jul 27 08:56:24 EST 1998


Hi All,

I am looking for reagents to add to plates that might reduce
the background observed in our 2-hybrid (LexA system) growth assays.
We are using LEU2 as our reporter.
(We note this problem only with a few constructs on -Leu plates;
and for these constructs LacZ as a reporter does work fine !).

Is there any reagent that, in practical terms, might work
for LEU2 similar to how 3-AT works for the HIS3 reporter gene
(i.e. some competitive inhibitor) ?

Can anyone help with info ? Many thanks !

Cheers,
         Rainer


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    Dr. Rainer Duden
    Department of Clinical Biochemistry,  University of Cambridge
    Addenbrooke's Hospital / Hills Rd.,  Cambridge CB2 2QR, England
    Office: +44-1223-762524
    Lab:    +44-1223-331960  (-- or leave message --)
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    E-mail: rd217 at cam.ac.uk
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