Hi All,
I am looking for reagents to add to plates that might reduce
the background observed in our 2-hybrid (LexA system) growth assays.
We are using LEU2 as our reporter.
(We note this problem only with a few constructs on -Leu plates;
and for these constructs LacZ as a reporter does work fine !).
Is there any reagent that, in practical terms, might work
for LEU2 similar to how 3-AT works for the HIS3 reporter gene
(i.e. some competitive inhibitor) ?
Can anyone help with info ? Many thanks !
Cheers,
Rainer
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Dr. Rainer Duden
Department of Clinical Biochemistry, University of Cambridge
Addenbrooke's Hospital / Hills Rd., Cambridge CB2 2QR, England
Office: +44-1223-762524
Lab: +44-1223-331960 (-- or leave message --)
Fax: +44-1223-330598
E-mail: rd217 at cam.ac.uk
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