In article (Dans l'article) <6pasop$b56 at net.bio.net>,
alain at lan1.molonc.mcgill.ca (Alain Nepveu) wrote (icrivait) :
> Dear colleagues,
> We have just performed a yeast two-hybrid screen. Some of the
> clones we pulled would make sense, however, there is a termination codon
> between the coding sequence of the activation domain and the long open
> reading frame of the (putative) interacting protein. To produce the fusion
> protein, ribosomes would have to skip over this termination codon. Is this
> possible? Someone from Clontech told us that this can happen, but I would
> like to get more feedback before we invest time analysing these clones.
> Thanks for your help.
>> Alain Nepveu
>alain at lan1.molonc.mcgill.ca
the easiest way is to do a western blot to check the size of the expressed
protein. They are now a lot of antibodies specific for activation domains
whatever the system you use.
Damien
