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Question

Damien hermand damien.hermand at fundp.ac.be
Sun Jul 26 17:15:54 EST 1998


In article (Dans l'article) <6pasop$b56 at net.bio.net>,
alain at lan1.molonc.mcgill.ca (Alain Nepveu) wrote (icrivait) :

> Dear colleagues,
>         We have just performed a yeast two-hybrid screen.  Some of the
> clones we pulled would make sense, however, there is a termination codon
> between the coding sequence of the activation domain and the long open
> reading frame of the (putative) interacting protein.  To produce the fusion
> protein, ribosomes would have to skip over this termination codon.  Is this
> possible? Someone from Clontech told us that this can happen, but I would
> like to get more feedback before we invest time analysing these clones.
> Thanks for your help.
> 
> Alain Nepveu
> alain at lan1.molonc.mcgill.ca

the easiest way is to do a western blot to check the size of the expressed
protein. They are now a lot of antibodies specific for activation domains
whatever the system you use.

Damien



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