Please refer to a comprehensive review by Zinser and Daum:
Yeast 11, 493-536. Isolation and biochemical characterization of
organelles from the yeast, Saccharomyces cerevisiae. (1995)
Hope this helps.
On 9 Jul 1998, Zen wrote:
> Date: 9 JUL 1998 08:34:23 -0700
> From: Zen <zen.lu at ikp.unibe.ch>
> Newgroups: bionet.molbio.yeast
> Subject: Membrane Isolation
>> Does somebody have a working protocol for isolating and solubilising
> membrane fraction from yeast?
>> Thank you
Program in Molecular Cancer Biology
Dept. of Pharmacology & Cancer Biology
Duke University Medical Center
Email: n.chung at duke.edu