>Does anyone know if nocodazole blocks S. pombe in metaphase?
>Are there any other reagents that could accomplish this?
Here is some information on microtubule binding drugs, which can be found
in the WWWeb version of a Fission Yeast Handbook at URL:
3. Arresting Cells with Drugs
Unlike S. cerevisiae, it is not possible to block S. pombe in mitosis with
microtubule binding drugs.
Thiabendazole is very effective at depolymerising microtubules in S. pombe
but does not cause a good cell cycle arrest, although there have been
reports that it can be used to block entry into mitosis.
Benomyl and MBC may be less effective in arresting the cell cycle, while
nocodazole is not effective.