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Hansenula polymorpha problem

Francisco P. Chavez Francisco.Chavez at cigb.edu.cu
Mon Jul 6 15:26:43 EST 1998

Dear Jonny Yokasawa
I don't know exactly the reason why Hansenula polymorpha grows well in YNB
medium, but normally in the routine work we use G0 medium (2 g/l NH4SO4, 6
g/l NH4H2PO4, 0.5 g/l MgSO4.7H2O, 0.1 g/l CaCL2, 0.1g/l NaCl, 0.1 g/l
KH2PO4, 0.02 g/l MgSO4.7H2O, 0.1 mg/l Na2MoO4.2H2O, 0.25 mg/l  H3BO3, 0.2
mg/l  ZnSO4.7H2O, 2 mg/l pyridoxine, 2 mg/l of Calcium pantetonate, 2 mg/l
of inosytol, 1 mg/l of  nicotinic acid, 0.02 mg/l of biotin, 20g/l of
glucose) and supplemented with 0.1 g/l of casein hydrolyze).
We also used in the fermentation experiment a modified medium reported by
Narciandi et al in 1995 (5g/l de KH2PO4, 4g/l de (NH4)2SO4, 0.5g/l KCl,
0.5g/l MgSO4 7H2O, 0.1g/l CaCl2 2H2O, 5 ml/l of a solution of traces
elements (2.01g/l ZnSO4 7H2O,0.02g/l CuSO4 5H2O, 0.083g/l KI, 0.304g/l MnSO4
H2O, 0.194g/l NaMoO4 H2O, 0.019g/l H3BO3,4.811g/l FeCl3 6H2O) and 10 ml of a
vitamine solution which contain 4 mg/l of calcium pantetonate, 1mg/l of
nicotinic acid, 4mg/l of tiamine-HCl, 4mg/l pyridoxine and 0.4mg/l of
biotine and 10 g/l casein hydrolyze.

G0 medium: Galzy  and Slonimsky (1957). Variation phisiologique de la levure
de la croissance sur l'acide lacctique comme seule source de carbone.
Comptes Rend. Acad. Sci. (Paris) 245, 2423.
Modified medium: Narciandi et al (1995) Biotechnology Letter 17, No 9.

Good luck

Francisco Chavez

Jonny Yokosawa wrote:

> Dear netters,
> I received a strain of Pichia angusta (formerly Hansenula polymorpha)from
> ATCC and want to use it to express heterologous protein.
> The catalog says that genotype is leu1 and ura3, but the strain grows well
> in YNB-URA, YNB-LEU or even YNB without any aminoacids. I wonder if
> anybody knows what is happening and how I can solve this problem.
> Thank you.
> Jonny Yokosawa
> jby6 at cdc.gov
> Centers for Disease Control and Prevention
> Atlanta, GA - USA

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