Dear Jonny Yokasawa
I don't know exactly the reason why Hansenula polymorpha grows well in YNB
medium, but normally in the routine work we use G0 medium (2 g/l NH4SO4, 6
g/l NH4H2PO4, 0.5 g/l MgSO4.7H2O, 0.1 g/l CaCL2, 0.1g/l NaCl, 0.1 g/l
KH2PO4, 0.02 g/l MgSO4.7H2O, 0.1 mg/l Na2MoO4.2H2O, 0.25 mg/l H3BO3, 0.2
mg/l ZnSO4.7H2O, 2 mg/l pyridoxine, 2 mg/l of Calcium pantetonate, 2 mg/l
of inosytol, 1 mg/l of nicotinic acid, 0.02 mg/l of biotin, 20g/l of
glucose) and supplemented with 0.1 g/l of casein hydrolyze).
We also used in the fermentation experiment a modified medium reported by
Narciandi et al in 1995 (5g/l de KH2PO4, 4g/l de (NH4)2SO4, 0.5g/l KCl,
0.5g/l MgSO4 7H2O, 0.1g/l CaCl2 2H2O, 5 ml/l of a solution of traces
elements (2.01g/l ZnSO4 7H2O,0.02g/l CuSO4 5H2O, 0.083g/l KI, 0.304g/l MnSO4
H2O, 0.194g/l NaMoO4 H2O, 0.019g/l H3BO3,4.811g/l FeCl3 6H2O) and 10 ml of a
vitamine solution which contain 4 mg/l of calcium pantetonate, 1mg/l of
nicotinic acid, 4mg/l of tiamine-HCl, 4mg/l pyridoxine and 0.4mg/l of
biotine and 10 g/l casein hydrolyze.
References
G0 medium: Galzy and Slonimsky (1957). Variation phisiologique de la levure
de la croissance sur l'acide lacctique comme seule source de carbone.
Comptes Rend. Acad. Sci. (Paris) 245, 2423.
Modified medium: Narciandi et al (1995) Biotechnology Letter 17, No 9.
942-952.
Good luck
Francisco Chavez
Jonny Yokosawa wrote:
> Dear netters,
>> I received a strain of Pichia angusta (formerly Hansenula polymorpha)from
> ATCC and want to use it to express heterologous protein.
> The catalog says that genotype is leu1 and ura3, but the strain grows well
> in YNB-URA, YNB-LEU or even YNB without any aminoacids. I wonder if
> anybody knows what is happening and how I can solve this problem.
> Thank you.
> Jonny Yokosawa
>jby6 at cdc.gov> Centers for Disease Control and Prevention
> Atlanta, GA - USA
