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Labelling protein under a GAL promoter

Pierre Belhumeur belhumep at ERE.UMontreal.CA
Tue Jul 7 00:34:40 EST 1998


Hi,

Does anyone have a straightforward protocol to S35 or P32 label a protein
which is under the control a GAL promoter? We are sort of familiar with the
different media to use, when the gene is under the control of its own
promoter but we'd like to know few hints when the gene is under a GAL
promoter (medium, time of induction, so forth...): a million thanks!


Pierre Belhumeur

Associate professor
Microbiology/Immunology
U of Montreal

belhumep at ere.umontreal.ca






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