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Joergen Hansen carlgaer at biobase.dk
Fri Jul 3 13:03:22 EST 1998


I do not know about Pichia, but for Saccharomyces yeasts, the only amino
acid that represses methionine formation efficiently is, methionine...It
would maybe also work to add S-adenosylmethionine, but this is very
expensive, and is converted to methionine anyway.

But why not just omit sulfate from your medium (use ammonium chloride
instead), and then add tiny amounts of methionine/cysteine for survival?

Best regards,

Joergen Hansen
carlgaer at biobase.dk

Katjusa Brejc wrote:

> Hi,
> we would like to incorporate selenomethionine instead of methionine
> into a
> particular protein expressed in yeast Pichia pastoris. Since our yeast
> strain is not a methionine auxotroph we would have to modified our
> medium in order to inhibit methionine biosynthesis.
> I would be very grateful if somebody could help me and tell which
> amino
> acids to add to inhibit Met synthesis.
> Thanks in advance
> Katjusa Brejc
> katjusa at nki.nl

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