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Hansenula polymorpha problem

Jonny Yokosawa jby6 at cdc.gov
Thu Jul 2 13:16:05 EST 1998

Dear netters,

I received a strain of Pichia angusta (formerly Hansenula
polymorpha)from ATCC and want to use it to express heterologous protein.
The catalog says that genotype is leu1 and ura3, but the strain grows
well in YNB-URA, YNB-LEU or even YNB without any aminoacids. I wonder if
anybody knows what is happening and how I can solve this problem.
Thank you.
Jonny Yokosawa
jby6 at cdc.gov
Centers for Disease Control and Prevention
Atlanta, GA - USA

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