Just a short question/request:
Does anybody know of a method to do random mutagenesis in budding yeast? If
possible with a TY/Lux (or GFP) fusion as a reporter. I am interested in
mutagenizing my yeast strain (as of yet an unidentified environmental
isolate) in order to identify promoters that are activated in response to
various stress conditions.
If those who read and respond to this request have the construct in hand I
would most appreciate it if you could contact me and send me the construct.
Thanks and I hope that I will get a few hits to my request.
Ron
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Ron Lahav
Environmental Microbiology
Jacob Blaustein Institute for Desert Research
Sde Boker Campus
Ben Gurion University of the Negev.
Beer Sheva, Israel.
Home e-mail: roni at lahavnet.co.il
Lab fax: -972-7-659-6831
Lab e-mail: ronil at bgumail.bgu.ac.il
