Does anybody have a S. cerevisiae genomic overexpression library with
appr. >7kb avg. size inserts and very little vector background?? I am
desperate for a good one.
In addition, does anyone know of a yeast transformation protocol that
transforms in larger plasmids? I have been using the standard LiAc
protocol and I find that vectors and smaller inserts are being
transformed in at a much, much higher rate then larger inserts. Any
help on either of these issues would be GREATLY appreciated.
Mike Liskay's lab
Oregon Health Sciences University
shelleye at ohsu.edu