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2 plasmids

Marc Fischer mff1 at york.ac.uk
Mon Jan 26 10:59:17 EST 1998

I am trying to co-express 2 proteins interacting as subunits in S. 
cerevisiae under the control of strong promoters (GAP and Gal1p). Each of 
these genes is carried by a plasmid with a different selection marker 
(leu or Ura). I have had some problems in obtaining the double 
transformant strain expressing the 2 genes. Has anyone had the same kind 
of problems with 2 different plasmids in the same cell and should I 
consider the integration of the genes into the genome instead of having 
my 2 genes as extrachromosomal information ?

mff1 at york.ac.uk

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