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Help with Pichia expression "green stuff"

Edwin Rydberg rydberg at chem.ubc.ca
Tue Jan 13 18:49:41 EST 1998


I am purifying Human Pancreatic alpha-Amylase, which we have expressed
in Pichia pastoris and I have had some problems removing the Green
substance.  I am aware of the low pH (5.5-6) cation exchange method of
purification, unfortunately my enzyme also fails to bind to a cation
exchange resin under these conditions.  Further more, I have not had
much success with dialysis or other membrane filtration methods.

Any help with this problem would be appreciated.


Edwin Rydberg
Dept. Biochem.  UBC






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