I am purifying Human Pancreatic alpha-Amylase, which we have expressed
in Pichia pastoris and I have had some problems removing the Green
substance. I am aware of the low pH (5.5-6) cation exchange method of
purification, unfortunately my enzyme also fails to bind to a cation
exchange resin under these conditions. Further more, I have not had
much success with dialysis or other membrane filtration methods.
Any help with this problem would be appreciated.
Dept. Biochem. UBC