Hong Xu wrote:
>> Dear Colleagues:
>> Our group is using the Clontech’s HeLa cDNA library in a yeast
> two-hybrid screening with the bait fused to the GAL4AD and the yeast
> strain CG 1945. Many of the clones sequenced show one of the following
> sequences, including ITBA2 (whatever it is, since almost no references
> can be found in Medline), ribosomal s20, transketolase, Ring12 (probably
> a proteasome subunit), ADP ribosylation factor, etc., with those listed
> above showing up most frequently. We would like to know whether or not
> these factors have also been found in somebody else’ two-hybrid
> screenings using the same Clontech HeLa library.
>> You are welcome to post your answer to our question in this newsgroup.
> Or you can call me at 973-781-7987.
>> Thank you very much.
>> Richard
Dear Richard,
try this URL and you find an overview of the most common trash found
in two hybrid screens:
http://www.fccc.edu/research/labs/golemis/main_false.html
Hope this helps,
Ricky
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Frederik Boernke
Research Group of Molecular Plant Physiologie
Institute for Plant Genetics and Crop Plant Research (IPK)
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Fax. 039482 -5 515
e-mail: boernke at ipk-gatersleben.dehttp://www.ipk-gatersleben.de
