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Potential nonspecific factors identified through a yeast

Frederik Boernke boernke at ipk-gatersleben.de
Wed Jan 7 09:10:38 EST 1998

Hong Xu wrote:
> Dear Colleagues:
> Our group is using the Clontech’s HeLa cDNA library in a yeast
> two-hybrid screening with the bait fused to the GAL4AD and the yeast
> strain CG 1945.  Many of the clones sequenced show one of the following
> sequences, including ITBA2 (whatever it is, since almost no references
> can be found in Medline), ribosomal s20, transketolase, Ring12 (probably
> a proteasome subunit), ADP ribosylation factor, etc., with those listed
> above showing up most frequently.  We would like to know whether or not
> these factors have also been found in somebody else’ two-hybrid
> screenings using the same Clontech HeLa library.
> You are welcome to post your answer to our question in this newsgroup.
> Or you can call me at 973-781-7987.
> Thank you very much.
> Richard

Dear Richard,

try this URL and you find an overview of the most common trash found
in two hybrid screens:


Hope this helps,


Frederik Boernke
Research Group of  Molecular Plant Physiologie
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
e-mail: boernke at ipk-gatersleben.de

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