hi, I am having trouble to detect protein in the range of 20-30 kd by
western blotting. I am tranferring the gel onto Nitrocellulose(0.22um)
as well as immobilon P using semi dry tranfer (biorad apparratus) and
the recommended voltage 10volt , 30min for a mini gel. upon transfer, by
ponceau staining , I could not detect sharply stained protein band. what
happen!
any help is wellcome
thanks
didier
