I have a S. cerevisiae strain expressing a c-myc epitope tagged protein.
Following cell wall removal and permeablisation with organic solvents I can
localise the protein by immunoperoxidase (HRP anti-mouse), but using a
SIGMA FITC anti-mouse the signal is poor. Any clues as to how to improve
the signal? I find I get high backgrounds with the avidin-biotin system
(SIGMA FITC-Avidin), so that isn't an option.
I could just stick with immunoperoxidase, but then I can't double label.
Anyone know of a *visible light* alternative to DAPI to stain nuclei?
Thanks
--
Fergus Doherty,
Biomedical Sciences,
Nottingham University,
Fergus.Doherty at nottingham.ac.uk
0115 970 9366 (74-41366 internal)
