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Yeast glycerol

Ron Lahav ronil at bgumail.bgu.ac.il
Mon Dec 21 07:28:30 EST 1998

Hi group, at the risk of sounding silly I have a question that I would like
to ask you.
Has anyone any experience using the Boehringer glycerol determination kit?
I know it sounds quite straight forward to use the kit, but I have had very
high variations in the results, including the control. I want to know if
maybe I am to blame for this or maybe the kit is defective, so I would
appreciate it if anyone who has extensive experience using this kit can
forward me their protocol from the stage of yeast cell disruption to the
final reading onthe spec. You know, a protocol "any 10 year old can do..".
Thanks in advance.

Ron Lahav
Environmental Microbiology
Jacob Blaustein Institute for Desert Research
Sde Boker Campus
Ben Gurion University of the Negev.
Beer Sheva, Israel.

Home e-mail: roni at lahavnet.co.il
Lab telephone: -972-7-659-6836
Lab fax: -972-7-659-6831
Lab e-mail: ronil at bgumail.bgu.ac.il

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