> From: Ge Qiang li <gxl29 at po.cwru.edu>
> I was looking at dominant negative effects of a mutant in haploid cells
> expressed under NMT control. But when I grew the transformants at 30
> and 37 degree centigrates, I found that all transformants including wt
> control died at 37 when plated on B1 containing plates. Can somebody
> come up with a theory to explain this observation?
It is certainly true that high temp is stressful to yeast cells. Our
usual maximum restrictive temp is 36*C. I don't think I've done much
with pombe at 37*C. The usual questions: is it dependent upon your
choice of wt strain or the media? If you are transforming, it is
probably safer to select transformants at lower temperature and then
replica plate or restreak to higher temperatures for your assay.
Hope this helps,
--
-susan
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