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pombe diploids will not sporulate!

Colin Rasmussen colin at nospam.fungus.usask.ca
Wed Dec 16 07:18:03 EST 1998


In article <755lb5$670 at net.bio.net>, Ge Qiang li <gxl29 at po.cwru.edu> wrote:

> I need to sporulate diploids for random spore analysis, I am growing
> them on selection media, and the colonies are sitting at RT for more
> than two weeks and there is no sporulation.  Any body knows what I can
> do to induce sporulation?

Put them on malt extract.  Are you sure there's no sporulation and what
you've got is now viable haploids.

Colin





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