To Whom It May Concern:
I am at the first time use <italic>S. pombe.</italic> I will perform
nuclear run-on. I have
1. Do sarcosyl equal N-lauroylsacosine (Sigma Cat. L9150, sodium salt)?
2.Can I use glass beads (425~600 microns, Sigma Cat. G9268 or G8772) to
isolate labeled RNA?
I would appreciate that you answer above questions.
Danyang Chen, M.D., Ph.D.
Department of Cell and Molecular Biology
Northwestern University Medical School
303 East Chicago Avenue
Ward Building, Rm. 11-240
Chicago, IL 60611
Tel: (312) 503 4276(Lab)
Fax: (312) 503 7912
E-mail: d-chen1 at nwu.edu