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[summary] Genealogy

Namjin Chung nc1 at acpub.duke.edu
Fri Aug 14 12:21:06 EST 1998

The following is summary about the origin of two popular Sc lab strains, 
S288C and W303

1. W303 (E-mail from Dr. Rothstein, the originator)

Date: Thu, 13 Aug 1998 16:43:00 -0400
From: Rodney Rothstein <rothstein at cuccfa.ccc.columbia.edu>
To: Namjin Chung <nc1 at acpub.duke.edu>
Subject: Re: W303 (fwd)

The construction of W303-1A and -1B is referenced in Thomas, B. J. &
Rothstein, R. (1989) Cell 56, 619-630.  The origin of this strain has a
long history.  It is somewhat of a "mutt."  Many crosses were made with
strains from my Ph.D. thesis, W87 derivatives (see Genetics 85: 35-54, 1977
and Genetics 85: 55-64, 1977).  These are mainly but not exclusively
S288c-like.  There is also genetic background from Fred Sherman's strains,
D311-3A thrown in for good measure (see Genetics 94: 871-889, 1980 and
Genetics 94: 891-898, 1980).  Finally, there is a real mutt which is one of
the grandparents of W303.  It is D190-9C, which I got from  Jack Szostak
about which very little is known.

I hope this is useful.

2. S288C

Date: Fri, 14 Aug 1998 14:28:46 +0000
From: Alan Boyd <AB at srv4.med.ed.ac.uk>
Reply to: alan.boyd at ed.ac.uk
To: nc1 at acpub.duke.edu
Subject: genealogy

Mortimer,RK, Johnston,JR, 1986 Genealogy of principal strains of the Yeast
Genetic Stock Center.
Genetics, 113,35-43

>From the above article, S288C also looks like another "mutt."  I'd be 
much happier if I could hear anything about sigma1278b strain, too.


   Namjin Chung
   Program in Molecular Cancer Biology
   Dept. of Pharmacology & Cancer Biology
   Duke University Medical Center
   Email: n.chung at duke.edu

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