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35-S Methionine labelling yeast cells?? S.pombe

Stuart Montgomery stuart.montgomery at wolfson.oxford.ac.uk
Wed Aug 12 05:45:50 EST 1998

Adrian Bracken wrote in message <35CB226A.6A6A at tcd.ie>...
>I am wondering if it is possible to 35-S Methionine label yeast cell
>proteins? I want to label cells, prepare extracts, immunoprecipitate a
>ribonuclear-protein complex and run the protein content out on an SDS
>gel. Because the protein content will not be detectable by
>silver-staining, I hope the Methinine labelled proteins will be
>visable after exposure to film.
>Do you know if this approach has been used before? If so, have you any
>recent publications on it?
>Please, if you could mail me at brackena at tcd.ie it would be great,
>Thanks a million, Adrian Bracken

I want to measure protein half life in S.pombe and have been told that you
can't use Methinine. Does anyone have any ideas?
Stuart Montgomery

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