I am wondering if it is possible to 35-S Methionine label yeast cell
proteins? I want to label cells, prepare extracts, immunoprecipitate a
ribonuclear-protein complex and run the protein content out on an SDS
gel. Because the protein content will not be detectable by
silver-staining, I hope the Methinine labelled proteins will be
visable after exposure to film.
Do you know if this approach has been used before? If so, have you any
recent publications on it?
Please, if you could mail me at brackena at tcd.ie it would be great,
Thanks a million, Adrian Bracken