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Ashok Aiyar aiyar at ebv.oncology.wisc.edu
Sat Aug 1 10:31:13 EST 1998

On 1 Aug 1998 07:28:48 -0700,
Aidan Weatherill (Aweather at worf.molbiol.ox.ac.uk) wrote:

>Specifically what concentration do people use for selecting transformants,
>and are there any special media that people use?

We use YEPD with 300 mg/L of zeocin to select for transformants.  We
passage the transformants on YEPD with 200 mg/L of zeocin.  When grown in 
liquid culture, we use YEPD with 300 mg/L of zeocin.

On synthetic dextrose plates, we have been using 150 mg/L of zeocin.

Ashok Aiyar, Ph.D.
McArdle Laboratory for Cancer Research
aiyar at ebv.oncology.wisc.edu

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