On 1 Aug 1998 07:28:48 -0700,
Aidan Weatherill (Aweather at worf.molbiol.ox.ac.uk) wrote:
>Specifically what concentration do people use for selecting transformants,
>and are there any special media that people use?
We use YEPD with 300 mg/L of zeocin to select for transformants. We
passage the transformants on YEPD with 200 mg/L of zeocin. When grown in
liquid culture, we use YEPD with 300 mg/L of zeocin.
On synthetic dextrose plates, we have been using 150 mg/L of zeocin.
Ashok Aiyar, Ph.D.
McArdle Laboratory for Cancer Research
aiyar at ebv.oncology.wisc.edu