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Protease inhibitor coctail for yeast?

Fergus Doherty Fergus.Doherty at nottingham.ac.uk
Wed Oct 29 12:17:34 EST 1997


I seem to be getting quite a lot of protein degradation in yeast
homogenates and subfractions (glass beads to break cells), even using PMSF,
leupeptin and pepstatin.  Sigma cell a cocktail of AEBSF (like PMSF?),
E-64, pepstatin A and o-phenanthroline (metal chelator?).  Is this any
good, and suggestions for a good cocktail.  If possible I would like to
leave out a calcium chelator - would this cause a problem?

Thanks

-- 
Fergus Doherty,
Dept Biochemistry,
Nottingham University,

Fergus.Doherty at nottingham.ac.uk
0115 970 9366 (74-41366 internal)






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