I seem to be getting quite a lot of protein degradation in yeast
homogenates and subfractions (glass beads to break cells), even using PMSF,
leupeptin and pepstatin. Sigma cell a cocktail of AEBSF (like PMSF?),
E-64, pepstatin A and o-phenanthroline (metal chelator?). Is this any
good, and suggestions for a good cocktail. If possible I would like to
leave out a calcium chelator - would this cause a problem?
Fergus.Doherty at nottingham.ac.uk
0115 970 9366 (74-41366 internal)