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pombe questions

Fred Kippert fkippert at srv0.bio.ed.ac.uk
Tue Oct 21 10:28:27 EST 1997

Steve  Ingram <ingram at uthscsa.edu> wrote:

> Two questions:
> 1. Does anyone know if the luciferase gene has been or can be used
> as a reporter in pombe? 2. What is the best source of the CAT gene
> to use as a reporter (by best I mean flanked by restriction sites
> useful for cloning)?

I am using firefly luciferase as a reporter for circadian rhythms. 
With the wild type gene, there a a couple of restrictions (such as 
stability of the protein, sensitivity of enzyme activity towards 
changes in the cytoplasmic environment. I have made constructs that 
largely overcome these problems, which would be available in a couple 
of weeks if you are interested. Bacterial luciferase is useless in 
both pombe and cerevisiae.

No idea about CAT.

Fred Kippert

Fred Kippert
Yeast Chronobiology Lab
Institute of Cell, Animal and Population Biology
University of Edinburgh
West Mains Road
Edinburgh EH9 3JT
Scotland, UK

Tel     0131 650 5488
FAX     0131 650 6564
e-mail  fkippert at srv0.bio.ed.ac.uk
URL:    http://helios.bto.ed.ac.uk/icapb/seminars/fyc/yeastchrono.html

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