Steve Ingram <ingram at uthscsa.edu> wrote:
> Two questions:
> 1. Does anyone know if the luciferase gene has been or can be used
> as a reporter in pombe? 2. What is the best source of the CAT gene
> to use as a reporter (by best I mean flanked by restriction sites
> useful for cloning)?
I am using firefly luciferase as a reporter for circadian rhythms.
With the wild type gene, there a a couple of restrictions (such as
stability of the protein, sensitivity of enzyme activity towards
changes in the cytoplasmic environment. I have made constructs that
largely overcome these problems, which would be available in a couple
of weeks if you are interested. Bacterial luciferase is useless in
both pombe and cerevisiae.
No idea about CAT.
Yeast Chronobiology Lab
Institute of Cell, Animal and Population Biology
University of Edinburgh
West Mains Road
Edinburgh EH9 3JT
Tel 0131 650 5488
FAX 0131 650 6564
e-mail fkippert at srv0.bio.ed.ac.uk