I'm seeking for recipes for SDS-Tricine buffers. I have checked with the
original paper by Schagger and Jagow, where they used different buffers
for anode, cathode, and gel. Could any one use single buffere for these?
What's the recipe, then? The original reciped have used 49.5%
acrylamide/3% bisacrylamide as gel stock solution; can I just use 30%
acrylamide/0.8% bisacrylamide, which are being popularly used.
Dept of Pharmacology & Cancer Biology
Duke University Medical Center
Email: n.chung at duke.edu