I am a graduate student in Microbiology using extracted _S. cerevisae_
DNA as part of a series of standard controls in dot-blot hybridizations,
have one (seemingly trivial) question.
One of the oligo probes which I am using is described as "Universal",
it does indeed bind to yeast genomic DNA (as well as mitochondrial, I
In the literature the probe's specificity is for the 16S rRNA coding region
(E. coli numbering). I am assuming that this particular sequence is
to a similar region in the 18S rRNA region of yeast...(?)
In any case, does anyone know how many binding sites in a single yeast
genome (approximately) would bind this probe assuming that it binds rRNA?
...or perhaps where I could discover this information?
Thanks in advance.
University of Georgia - Microbiology
dsingle at uga.cc.uga.edu