IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

Post-doc position in protein expression and labelling

Dominique MARION marion at ibs.fr
Fri Oct 17 03:01:12 EST 1997

    The NMR laboratory of the "Institut de Biologie Structurale" Jean-Pierre
Ebel in Grenoble (France) is involved in the solution structure determination of
proteins using nuclear magnetic resonance. Uniformly labelled proteins (15N and
13C) are produced by overexpression either in E. coli or in yeast and studied by
multidimensional NMR spectroscopy.

    A post-doctoral scientist is required to develop protein expression in our
laboratory.  A strong background in both molecular biology (cloning, PCR ...)
and protein biochemistry is required to elaborate high-yield protein expression
and purification techniques at the genetic and biochemical level.  Experience
with the expression of recombinant or epitope-tagged fusion proteins (poly-His,
GST, MalE) and refolding techniques for their purification from inclusion bodies
would be an advantage. The candidate should be familiar with techniques used for
protein characterization (optical spectroscopy,
CD...) but not necessarily with sophisticated NMR methods.  Because of the
expensive cost of the 15N/13C labelling, obtention of high yield of proteins is
the major issue.

    The current interest of the NMR laboratory focuses on several types of
proteins, such as enzymes from plant photorespiratory chains, proteins of the
membrane integrity of E.coli, or cell adhesion proteins.  Each project is
developped in close collaboration with a biology team in Grenoble or elsewhere.

    Financial support is already available for a first year and might be
extended to several years.  Applicants from the European community (except
french citizens) are invited to send a detailed CV with publications by mail
(before December 15th) to the following address:

Dominique MARION
Institut de Biologie Structurale Jean-Pierre Ebel
C.N.R.S - C.E.A. -- 41, Avenue des Martyrs
38027 Grenoble Cedex 1 - France

More information about the Yeast mailing list

Send comments to us at biosci-help [At] net.bio.net