Does anyone have any experience with expression in S. pombe coming
through the pART1 polylinker in the opposite orientation from the adh
promoter? We have gene that we believe is normally expressed at very
low levels. Our subclone into pART1 gave better complementation in the
"wrong" orientation than in the adh-driven orientation. Other data
suggests that this is not a case of "we are looking at the wrong ORF".
"I'd rather be fission."