Dear Dr. Aranda,
in Plant Journal 11 (6) 1997, 1237-1251 the group of Dr. Terry Thomas
used an optimized one-hybrid system to find some DC3 binding proteins.
A better HIS3 reporter plasmid "was prepared by replacing the HIS3 gene
of pRS315HIS with a more sensitive one" (whatever this means).
Agustin Aranda wrote:
>> I am obtaining many false positives in my one-hybrid screening. Does
> anybody know any tips to avoid this? Thanks,
>> Agustin Aranda
Institute of Genetics
University of Hohenheim
Emil Wolff Str.14