I am going to use sequence specific primer (instead of random hexamer
primer) to initiate retrotranscription in RT-PCR. I would like to share the
information of efficient condition (like primer concentration, annealing
and extension temperature and time). If you happen to know this or some
reference source, please e-mail me. Your attention and time is appreciated.
Thanks and have a nice day!
Yuxing Li
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Yuxing Li, Post-doctoral Associate
1144 Vet. Med. Complex phone : (515)-294-9882
Iowa State University email: yxl at iastate.edu
Ames, IA 50011, USA fax : (515)-294-8500
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